CRISPR Core & Resources

 

The UCI SCRC CRISPR Core provides CRISPR gene editing services to targeted gene knock-out or knock-in/point mutation lines. Gene editing may be performed in human iPSC/ESC/NSC supplied by the core facility or your laboratory (referred to as user “user cell line”). Immortalized cell lines the core can purchase from ATCC may also be used. The following services are offered.

* Allele-specific analysis is offered for an additional fee. Please inquire.
For questions, please contact scrc-crispr@uci.edu 

Request Service

This service typically takes 2-4 weeks and includes:

• Consultation to discuss optimal CRISPR design and targeting strategy.
• 1-2 gRNAs, sequence-verified for on-target activity (sequencing results included).
• List of top 10 predicted off-target sites.

 

This service typically takes 2-3 months and includes:

• Consultation
• gRNA design and validation
• Delivery of gRNA and Cas9 into one nominated cell line
• Pre-screening of cell pool
  • Bank cell pool
• Expansion and sequence validation of up to 3 modified clones
• Bank expanded clones
• Characterization of modified clones
  • Sterility/mycoplasma testing for all lines
  • Karyotype assessment for all non-immortalized lines
  • Pluripotency assessment for all hiPSC/ESC lines

Deliverables include:

• Two vials of one genetically modified line
  • Additional vials may be requested for $80 per vial
  • Additional clones may be requested for $800 per clone
• Sequence data of validated clones
• All characterization reports, performed as indicated

 This service typically takes 2-3 months and includes:

• Consultation
• gRNA design and validation
• Design and generation of donor template
• Delivery of gRNA and Cas9 into one nominated cell line
• Pre-screening of cell pool
  • Bank cell pool
• Expansion and sequence validation
• Bank expanded clones (5 vials per line)
• Characterization of modified clones
  • Sterility/mycoplasma testing for all lines
  • Karyotype assessment for all non-immortalized lines
  • Pluripotency assessment for all hiPSC/ESC lines

Deliverables include:

• Two vials of one genetically modified line
  • Additional vials may be requested for $80 per vial
  • Additional clones may be requested for $800 per clone
• Sequence data of validated clones
• All characterization reports, performed as indicated

 

 This service typically takes 3-5 weeks and includes:

• Sterility/mycoplasma testing
• Karyotype determination
• Pluripotency testing for hiPSC/ESC lines
• Transfection optimization

All characterization reports will be provided

Addgene CRISPR Overview and Guide

Invitrogen CRISPR Search and Design Tool Guide

Chromas: DNA sequencing viewer

Snapgene Viewer: DNA sequencing viewer

NCBI Gene: Refseq/ ENSEMBL/gene ID search

The Human Protein Atlas

Ensemble Genome Browser

ChopChop: gRNA design tool

DKFZ: German Cancer Research Center CRISPR Design Tool

MIT CRISPR Design: gRNA search and off-target Analysis

Codon Analysis: Frequency of Amino Acid Codons per gene input

GenScript Codon Usage Frequency Table Tool: Search by species

CRISPR RGEN Tools: RNA Guided Endonuclease Database