SCRC

Gene Editing Services

Section 1

Gene Editing Service

University of California Rates

Time

gRNA Design and Validation

$750

2-4 weeks

Targeted Gene Knock-Out

$7,500

4-6 months

Targeted Gene Knock-In/Point Mutation or Reporter Line

$8,500

5-7 months

User Cell Line Characterization

$1,200

3-5 weeks

gRNA Design And Validation

This service typically takes 2-4 weeks and includes:

  • Consultation to discuss optimal CRISPR design and targeting strategy.
  • 1-2 gRNAs, sequence-verified for on-target activity (sequencing results included).
  • List of top 10 predicted off-target sites.
 

Target Gene Knock-Out

This service typically takes 2-3 months and includes:

  • Consultation
  • gRNA design and validation
  • Delivery of gRNA and Cas9 into one nominated cell line
  • Pre-screening of cell pool
    • Bank cell pool
  • Expansion and sequence validation of up to 3 modified clones
  • Bank expanded clones
  • Characterization of modified clones
    • Sterility/mycoplasma testing for all lines
    • Karyotype assessment for all non-immortalized lines
    • Pluripotency assessment for all hiPSC/ESC lines

Deliverables include:

  • Two vials of one genetically modified line
    • Additional vials may be requested for $80 per vial
    • Additional clones may be requested for $800 per clone
  • Sequence data of validated clones
  • All characterization reports, performed as indicated

Targeted Gene Knock-In/Point Mutation

 This service typically takes 2-3 months and includes:

  • Consultation
  • gRNA design and validation
  • Design and generation of donor template
  • Delivery of gRNA and Cas9 into one nominated cell line
  • Pre-screening of cell pool
    • Bank cell pool
  • Expansion and sequence validation
  • Bank expanded clones (5 vials per line)
  • Characterization of modified clones
    • Sterility/mycoplasma testing for all lines
    • Karyotype assessment for all non-immortalized lines
    • Pluripotency assessment for all hiPSC/ESC lines
Deliverables include:
  • Two vials of one genetically modified line
    • Additional vials may be requested for $80 per vial
    • Additional clones may be requested for $800 per clone
  • Sequence data of validated clones
  • All characterization reports, performed as indicated
 

User Cell Line Characterization

 This service typically takes 3-5 weeks and includes:

  • Sterility/mycoplasma testing
  • Karyotype determination
  • Pluripotency testing for hiPSC/ESC lines
  • Transfection optimization
All characterization reports will be provided

Project Workflow

GeneWorkFlow