SCRC

Zeiss LSM 900 Airyscan 2/ GaAsP Confocal Multiplex Microscope

Zeiss LSM 900 Airyscan 2/ GaAsP Confocal Multiplex Microscope

The Zeiss LSM 900 Airyscan 2/GaAsP Confocal Multiplex system comes with AI Sample Finder for automated sample identification for efficient imaging. The system includes 405nm, 488nm, 561nm, and 640nm solid-state lasers, Airyscan 2 for super resolution imaging, 2x GaAsP-PMTs for confocal imaging, cohesive contrast, as well as temperature/CO2/humidity control stage though a stage-top incubator and an incubated chamber for the microscope.

Airyscan vs Confocal:

Classic confocal laser scanning microscopes use point illumination to scan the sample sequentially. The microscope optics transform each point to an extended Airy disk (Airy pattern). A pinhole then spatially limits this Airy disk to block out-of-focus light from reaching the detector. Closing the pinhole gives higher resolution, but at the price of detecting fewer photons – and these photons cannot be brought back by e.g. deconvolution.

Airyscan 2 is an area detector with 32 concentrically arranged detection elements. This allows you to acquire more of the Airy disk at once. The confocal pinhole itself remains open and does not block light, thus more photons are collected. This produces much greater light efficiency while imaging. Airyscan 2 gives you a unique combination of gentle superresolution imaging and high sensitivity.

The objectives include:

  • EC Plan-Neofluar 2.5x/0.085 M27 (FWD=8.8mm)
  • Plan-Apochromat 10x/0.45 M27 (FWD=2.1mm)
  • Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm)
  • Plan-Apochromat 40x/1.2 (FWD=0.41mm at CG=0.17mm) Imm Corr DIC M27 for water or glycerine immersion (CG=0.15-0.19mm)
  • Plan-Apochromat 63x/1.4 Oil DIC M27 (FWD=0.19mm)

The recharge rate has not yet been established and is currently available to use at no charge. This will be updated within the year.

For Training Please Contact:

Allia Fawaz
SCRC Core Microscope Imaging Manager
Gross Hall Building Manager
(949) 824-0430