SCRC

Zeiss LSM510 Confocal 2-Photon Fluorescent Inverted Microscope

ZEISS LSM510 CONFOCAL 2-PHOTON FLUORESCENT INVERTED MICROSCOPE

The LSM510 includes Argon Ion, HeNe, and 2-photon Coherent lasers, as well as a stage top incubator.  The objectives include:

  • EC Plan-Neofluar 10x/0.30
  • Plan-Apochromat 20x/0.8
  • EC Plan-Neofluar 40x/1.300il
  • Plan-Apochromat 63x/1.40il DIC
  • empty position
  • EC Plan-Neofluar 5x/0.15

Traditional fluorescent light microscopy uses epi-fluorescence to illuminate the entire field.  The LSM510 confocal microscope uses a laser 1-photon or 2-photon point light source.  The advantage of confocal microscopy is the usage of a spatial pinhole to block out-of-focus light in the z-axis above and below the focal plane creating less blur and better spatial resolution.  The additional advantage of a 2-photon light source is reduced tissue damage caused by photo-bleaching, deeper tissue penetration due to reduced light scatter of red light in tissue (compared to shorter wavelength light), and higher specificity of fluoresced light detection, effectively resulting in better spatial resolution.

The advantage of a single pinhole confocal over a spinning disk microscope is increased spatial resolution.  The advantage of a spinning disk microscope over a single pinhole confocal is increased time resolution at the potential expense of spatial resolution.

SCRC Core equipment fluorescent filter EX_EM wavelengths.pdf

Training: 

Zeiss LSM 510 training video (78 min)

Zeiss LSM 510 clean-up video (7 min)

LSM510_Quickstart_poster_SCRC

Confocal config guide_v1.2.pdf

Contact:  Christina Tu, Core Facility Coordinator, christina.tu (at) research.uci.edu

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