SCRC Core Facility Guidelines

SCRC Core Facility Guidelines


December 2010


EH&S Courses

  • Complete Laboratory Core Safety/ Hazardous Waste/ Blood borne Pathogens/ (Viral Vector)


hSCRO & IBC Approvals

  • Provide copy of approvals indicating Core as one location and names of personnel


Safety Regulations

  • No food or drink; wear closed toe shoes


Tissue Culture Room

  • Shower prior; wear clean clothes
  • Step firmly on the sticky pad at the entrance of the room; use shoe covers if needed
  • Wear lab coat designated for the tissue culture room only, remove and hang it on the hook upon leaving the room
  • Wear gloves; Tie long hair; Wear mask in flu and allergy season or as needed


CO2 Incubator

  • Product manual is avaliable
  • Wear gloves to handle cultures
  • When the light indicating RH PAN is on, add Arrowhead distilled water to the tray
  • Wipe clean any spill as soon as possible and clean with Bacdown detergent
  • Close door gently and wait for CO2 to come back to 5% before leaving
  • Check CO2 source frequently; report when tanks are empty; discard any old cultures


Bio-Safety Hood

  • Product manual is available
  • Turn on hood by raising sash and turning on fan;Spray entire hood space with 70% ethanol thoroughly and wait for 3 minutes warm up time; Align sash if needed
  • Keep only necessary items (pipettes, pipette aid and racks) in the hood; Put away your own items (tips) to avoid cross-contamination from sharing
  • Wipe clean any spill with 2% Bacdown detergent; if the spill goes under the panel, clean up by lifting up the panel and wiping it clean; recharge the pipette aid as needed
  • Upon finishing, rinse vacuum line with BD detergent before turning off the vacuum and wipe area clean with BD detergent including rim and sash, turn off hood by turning off fan and close sash



  • Spray and wipe tubing with 70% ethanol and bring into the hood; always run on vacuum before connecting to aspirating pipet to avoid back flow of air
  • After usage of the hood, rinse inside of tubing with 2% Bacdown detergent (not ethanol) and hang it on the hook outside of the hood; shut off vacuum


Empty the Receiver Bottle Frequently

  • Empty liquid waste through a strainer in the sink
  • Discard solid in trash
  • Rinse clean and fill the receiver with at least 60ml Bacdown detergent
  • Clean up sink and strainer


Water Bath

  • Refill with Millipore water if the water level is low.
  • Squirt some detergent into the water bath.
  • If there are any spills into the water bath, empty, clean and refill with water.


Red Container Trash Bin and Blue Recycle Bin

  • Deposit lab wares that come in contact with human cultures (pipettes, tips or plates that touch the human cells…..). Aspirate liquid before discarding.
  • If the red container is full, tie and place the red bag into the big red container outside of the tissue rooms (Do not over-fill the big container). Replace with new red bag.
  • Any other (empty containers, pipettes or tips that transfer un-used medium) can be disposed into the trash bins.
  • Re-sheath serological pipets back to envelope before discarding to red or trash bins
  • Medium bottles that have not touch any cells may discard in the recycle bin



  • Please wipe clean any spill or dirt and pick up tips, parafilm and any trash.Swiffer sweeper is available for wiping.
  • Please keep the floor area as clean as possible!


Packing Boxes

  • Flatten boxes and place them in the area outside 1401


Refrigerator and Freezer

  • Discard old media
  • Be organized


Liquid Nitrogen Storage

  • Face shield, thermal gloves and closed-toe shoes are required. Do not place the lid or rack on the floor, instead, place them on the cart.
  • Use black ruler to confirm level of LN


Emergency Numbers

  • 911 = UCI Police; if any emergency situation arises that requires 911 assistance, please call 911, which will connect to UCI Police UCI Facilities 824-5444