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A CIRM INSTITUTE
From the Director
Sue and Bill Gross
Faculty, Gross Hall
Faculty, UCI Campus
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SCRC Core Facility Guidelines
SCRC Core Facility Guidelines
Complete Laboratory Core Safety/ Hazardous Waste/ Blood borne Pathogens/ (Viral Vector)
hSCRO & IBC Approvals
Provide copy of approvals indicating Core as one location and names of personnel
No food or drink; wear closed toe shoes
Tissue Culture Room
Shower prior; wear clean clothes
Step firmly on the sticky pad at the entrance of the room; use shoe covers if needed
Wear lab coat designated for the tissue culture room only, remove and hang it on the hook upon leaving the room
Wear gloves; Tie long hair; Wear mask in flu and allergy season or as needed
Product manual is avaliable
Wear gloves to handle cultures
When the light indicating RH PAN is on, add Arrowhead distilled water to the tray
Wipe clean any spill as soon as possible and clean with Bacdown detergent
Close door gently and wait for CO2 to come back to 5% before leaving
Check CO2 source frequently; report when tanks are empty; discard any old cultures
Product manual is available
Turn on hood by raising sash and turning on fan;Spray entire hood space with 70% ethanol thoroughly and wait for 3 minutes warm up time; Align sash if needed
Keep only necessary items (pipettes, pipette aid and racks) in the hood; Put away your own items (tips) to avoid cross-contamination from sharing
Wipe clean any spill with 2% Bacdown detergent; if the spill goes under the panel, clean up by lifting up the panel and wiping it clean; recharge the pipette aid as needed
Upon finishing, rinse vacuum line with BD detergent before turning off the vacuum and wipe area clean with BD detergent including rim and sash, turn off hood by turning off fan and close sash
Spray and wipe tubing with 70% ethanol and bring into the hood; always run on vacuum before connecting to aspirating pipet to avoid back flow of air
After usage of the hood, rinse inside of tubing with 2% Bacdown detergent (not ethanol) and hang it on the hook outside of the hood; shut off vacuum
Empty the Receiver Bottle Frequently
Empty liquid waste through a strainer in the sink
Discard solid in trash
Rinse clean and fill the receiver with at least 60ml Bacdown detergent
Clean up sink and strainer
Refill with Millipore water if the water level is low.
Squirt some detergent into the water bath.
If there are any spills into the water bath, empty, clean and refill with water.
Red Container Trash Bin and Blue Recycle Bin
Deposit lab wares that come in contact with human cultures (pipettes, tips or plates that touch the human cells…..). Aspirate liquid before discarding.
If the red container is full,
and place the red bag into the big red container outside of the tissue rooms (Do not over-fill the big container). Replace with new red bag.
Any other (empty containers, pipettes or tips that transfer un-used medium) can be disposed into the trash bins.
Re-sheath serological pipets back to envelope before discarding to red or trash bins
Medium bottles that have not touch any cells may discard in the recycle bin
Please wipe clean any spill or dirt and pick up tips, parafilm and any trash.Swiffer sweeper is available for wiping.
Please keep the floor area as clean as possible!
Flatten boxes and place them in the area outside 1401
Refrigerator and Freezer
Discard old media
Liquid Nitrogen Storage
Face shield, thermal gloves and closed-toe shoes are required. Do not place the lid or rack on the floor, instead, place them on the cart.
Use black ruler to confirm level of LN
911 = UCI Police; if any emergency situation arises that requires 911 assistance, please call 911, which will connect to UCI Police UCI Facilities 824-5444