test1
iPSC Culturing Core
Core Leadership and Personnel
Christina Tu brings over 19 years of expertise to the forefront of induced pluripotent stem cell research and education. As the Director of the iPSC Culturing core facility, she combines technical mastery with leadership in advancing stem cell technologies and training the next generation of researchers. Her innovative teaching has trained over 600 participants in cutting-edge iPSC techniques.
Contact
Please contact Christina Tu at htu@uci.edu
Courses
iPSC Culturing Techniques Hands-on Course
Human pluripotent stem cell culture techniques including passaging, cryo-preserving and thawing, embryoid body formation, transfection, cardiomyocyte differentiation and neuronal progenitor differentiation.
Duration: two weeks, M-F 1:00 to 5:00pm
Dates: contact Christina Tu htu@uci.edu
Tuition: UCI $1,745; non-UCI: $3,490
Curriculum
Culturing
Passaging & Maintaining
Routine passaging and maintenance are the most vital techniques. Having good quality cells is a crucial element for successful differentiations. We will be practicing a few times in passaging and daily in maintaining iPS cells, in various subtracts and media. We will also talk about problem solving. Please be reminded that this is the most crucial techniques!
Freezing & Thawing
Stem Cells can be cryo-preserved for years. Proper techniques assure their viability.
Mechanical Selection
Manually collecting colonies or removing unwanted cells and are useful in maintenance, differentiations, and reprogramming.
Differentiation
Embryoid Body (EB) Formation
Many differentiation protocols start with forming EB formation. We will do size-controlled EB formation.
Neuronal Progenitors and Spontaneously differentiation (via EB)
In neuronal induction media, cells in formed EBs are directed toward neuronal lineage or in regular media for spontaneous differentiations.
Cardiomyocytes
A commercial kit will be used for generating cardiomyocytes. If done well, cultures having cells beating in rhythm can be observed.
Hematopoietic Progenitor Cells (offered in some courses)
A commercial kit will be used for generating Hematopoietic Progenitor Cells
Modification
GFP Transfection
Lonza nucleofection (electroporation) is used to introduce GFP plasmid for GFP expression. Key step in CRISPR editing
iPSC Reprogramming Hands-on Course
iPSC basic culturing techniques plus reprogramming techniques including fibroblast seeding, transduction of Yamanaka Factors, and clone selection.
Duration: expecting lasts up to two months
Date: required sufficient applicants to start a course; contact Christina Tu htu@uci.edu
Tuition: UCI $1,745; non-UCI: $3,490
(Photos from current website until new ones are available)