SCRC

Flow Cytometry Core

Section 1

Fluidigm Helios (a CyTOF® system) Mass Cytometer - Available Fall 2019

  • CyTOF® technology enables deep profiling of translational and clinical research sample across a range of cell surface and intracellular markers
  • Cell suspension analysis using antibodies conjugated to metal isotopes
  • Ability to analyze over 40 parameters on single cells via time-of-flight mass spectrometer detection

Stem Cell Flow Core Equipment listing and UCI academic pricing BD AriaII Cell Sorter

  • Cell sorter has four lasers (405nm, 488nm, 561nm, 640nm) and can detect up to 13 color parameters. Equipped with a moveable platform for clone sorting into 96 well plates, and a temperature controller for maintaining sorted cells at cold temperatures during sorts.
  • $80 per hour operator only
  • Gross Hall room 1302

  BD Fortessa flow cytometer
  • Cell analyzer with five lasers (355nm, 405nm, 488nm, 561nm, 640nm) and can detect up to 18 color parameters. It is equipped with a BD High Throughput Sampler for rapid analysis of samples prepared in plate formats.
  • $40 per hour operator use/ $60 per hour assisted use
  • Gross Hall bay 1200

BD Fusion Cell Sorter
  • Cell sorter has five lasers (355nm, 405nm, 488nm, 561nm, 640nm) and can detect up to 18 color parameters. It is equipped with a moveable platform for clone sorting into 96 well plates, and a temperature controller for maintaining sorted cells at cold temperatures during sorts, and is kept in a BSL2 biosafety cabinet for sterile cell sorting.
  • $80 per hour operatory only
  • Gross Hall room 1313

  BD LSRII flow cytometer
  • Cell analyzer with three lasers (488nm, 561nm, 640nm) and can detect up to 10 color parameters.
  • $40 per hour operator use/ $60 per hour assisted use
  • Gross Hall room 1201

  Miltenyi AutoMACS; Automatic Magnetic bead cell sorter
  • Fully automated magnetic bead cell sorter housed in sterile biosafety hood
  • No cost
  • Gross Hall 1401

Stem Cell Flow Core administers DeNovo Software FCS Express Data Analysis site license, for information about purchasing an annual seat email: Vanessa Scarfone vanessa.s@uci.edu and register for FCS Express account.