SCRC

Flow Cytometry Core

Section 1

Fluidigm Helios (a CyTOF® system) Mass Cytometer

HeliosThe FLUIDIGM Helios is a high-performance cell analyzer providing multiparameter analysis, including simultaneous detection of > 50 distinct parameters. This system employs probes (most often antibodies) that are labeled with heavy metals to be detected by time of flight (TOF) mass spectrometry.

  • CyTOF® technology enables deep profiling of translational and clinical research samples across a range of cell surface and intracellular markers
  • Cell suspension analysis using antibodies conjugated to metal isotopes
  • Gross Hall room 1401

SCRC Metal Conjugated Antibody Bank

To increase accessibility and reduce cost, the SCRC is generating an antibody bank for use with our mass cytometry technology. Products and services include:

Service Description

UC

Non-UC

HELIOS instrument usage (staff operated per-hour)

$125

$225

Custom probe metal conjugation (customer provides antibody)

$155

$310

Antibody bank reagent pre-conjugated (per sample)

$10

$20

Sample barcoding (per sample)

$35

$70

CYTOF Data Analysis (per hour)

$125

$250


Stem Cell Flow Core Equipment listing and UCI academic pricing BD AriaII Cell Sorter

  • Cell sorter has four lasers (405nm, 488nm, 561nm, 640nm) and can detect up to 13 color parameters. Equipped with a moveable platform for clone sorting into 96 well plates, and a temperature controller for maintaining sorted cells at cold temperatures during sorts.
  • $80 per hour operator only
  • Gross Hall room 1302

  BD Fortessa flow cytometer
  • Cell analyzer with five lasers (355nm, 405nm, 488nm, 561nm, 640nm) and can detect up to 18 color parameters. It is equipped with a BD High Throughput Sampler for rapid analysis of samples prepared in plate formats.
  • $40 per hour operator use/ $60 per hour assisted use
  • Gross Hall bay 1200

BD Fusion Cell Sorter
  • Cell sorter has five lasers (355nm, 405nm, 488nm, 561nm, 640nm) and can detect up to 18 color parameters. It is equipped with a moveable platform for clone sorting into 96 well plates, and a temperature controller for maintaining sorted cells at cold temperatures during sorts, and is kept in a BSL2 biosafety cabinet for sterile cell sorting.
  • $80 per hour operatory only
  • Gross Hall room 1313

  BD LSRII flow cytometer
  • Cell analyzer with three lasers (488nm, 561nm, 640nm) and can detect up to 10 color parameters.
  • $40 per hour operator use/ $60 per hour assisted use
  • Gross Hall room 1201

  Miltenyi AutoMACS; Automatic Magnetic bead cell sorter
  • Fully automated magnetic bead cell sorter housed in sterile biosafety hood
  • No cost
  • Gross Hall 1401


Stem Cell Flow Core administers DeNovo Software FCS Express Data Analysis site license, for information about purchasing an annual seat email: Vanessa Scarfone vanessa.s@uci.edu and register for FCS Express account.