SCRC

Boiko, Alexander D., Ph.D.

Alexander D. Boiko, Ph.D.

Assistant Professor

Biography
B.S., University of Illinois at Chicago, Chicago IL, 1997, Biology Ph.D., University of Illinois at Chicago, College of Medicine, Department of Molecular Genetics, Chicago IL, 2005, Molecular and Cancer Biology

Research Focus
Cancer, Stem Cells, Tumor Initiating Cells, Melanoma, Neural Crest

Appointments
Postdoctoral Scholar, Laboratory of Irving Weissman, Stanford University, Institute for Stem Cell Biology and Regenerative Medicine

Research Abstract
Research in Lay Terms Our Lab is interested in understanding cellular and molecular origins of melanoma initiation and clonal progression, as well as, to determine factors underlying aggressive metastatic properties of this disease.

Research Focus Dr. Boiko’s Lab focuses on discovering key molecular differences between tumor initiating and differentiated cell populations using human surgical melanoma samples. In addition, they are studying involvement of the immune system in control of disease progression and how to mediate its components to design more efficient therapeutic regimens.

Detail on Research The goal of our research is to identify molecular factors and cell lineages of neural crest origin that contribute to melanoma initiation. We are using gene expression analysis to pinpoint signaling networks that are circumvented during normal development or regeneration of melanocytic lineages and result in malignant melanoma transformation. Using RNA inhibition and cDNA overexpression approaches we modulate expression of the candidate genes in target cell populations that are then assayed for their tumorigenic properties in-vitro and in-vivo. Dr. Boiko lab is also interested in understanding the role of macrophages in melanoma progression and metastasis. Currently we are developing tools that modulate macrophage activity and testing their effect in-vivo in the mouse xenotransplantation model of human metastatic disease.

Publications
Boiko AD “Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues” Methods Mol Biol. 2013;961:253-9.

Razorenova OV, Finger EC, Colavitti R, Chernikova SB, Boiko AD, Chan CK, Krieg A, Bedogni B, LaGory E, Weissman IL, Broome-Powell M, Giaccia AJ. VHL loss in renal cell carcinoma leads to up-regulation of CUB domain-containing protein 1 to stimulate PKC{delta}-driven migration. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1931-6.

Boiko AD, Razorenova OV, van de Rijn M, Swetter SM, Johnson DL, Ly DP, Butler PD, Yang GP, Joshua B, Kaplan MJ, Longaker MT, Weissman IL. Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271. Nature. 2010 Jul 1;466(7302):133-7.

Boiko AD, Porteous S, Razorenova OV, Krivokrysenko VI, Williams BR, Gudkov AV. A systematic search for downstream mediators of tumor suppressor function of p53 reveals a major role of BTG2 in suppression of Ras-induced transformation. Genes Dev. 2006 Jan 15;20(2):236-52.

Lu T, Burdelya LG, Swiatkowski SM, Boiko AD, Howe PH, Stark GR, Gudkov AV. Secreted transforming growth factor beta2 activates NF-kappaB, blocks apoptosis, and is essential for the survival of some tumor cells. Proc Natl Acad Sci U S A. 2004 May 4;101(18):7112-7.

Zou X, Ray D, Aziyu A, Christov K, Boiko AD, Gudkov AV, Kiyokawa H. Cdk4 disruption renders primary mouse cells resistant to oncogenic transformation, leading to Arf/p53-independent senescence. Genes Dev. 2002 Nov 15;16(22):2923-34.

Axenovich SA, Kazarov AR, Boiko AD, Armin G, Roninson IB, Gudkov AV. Altered expression of ubiquitous kinesin heavy chain results in resistance to etoposide and hypersensitivity to colchicine: mapping of the domain associated with drug response. Cancer Res. 1998 Aug 1;58(15):3423-8.

Professional Societies
American Association of Cancer Research International Society of Stem Cell Research

Graduate Programs
Cancer Biology Cell Biology Cellular and Molecular Biosciences Developmental Biology and Genetics Mechanisms of Gene Expression Stem Cell Biology

Research Centers Chao Family Comprehensive Cancer Center Stem Cell Research Center

Department Affiliations Molecular Biology and Biochemistry School of Biological Sciences

Additional Links http://www.faculty.uci.edu/profile.cfm?faculty_id=5951